CFAR Viral Pathogenesis and Persistence

Overview of Services

CFAR Viral Pathogenesis and Persistence Core supports research on HIV and associated pathogens, by providing state-of-the-art biosafety laboratories, with flow cytometry, microscopy, and next generation sequencing equipment. The Core provides an efficient, cost-effective and safe environment for researchers involved in HIV research, in its BSL-3 and BSL-2+ facilities. The Core offers facilities for growth and analysis of microbial and viral pathogens together with training in methodologies of HIV culture and assay. Users of the BSL-3 facility must be certified and individual projects approved by the BSL-3 Advisory Group. The Core facilities are located on the 10th floor of the Biomedical Research Building and 2nd floor of the Wood Research Tower. The Core also provides access and expertise to next-generation sequencing technology in a CLIA/CAP accredited environment.

Equipment

DeltaVision Core Deconvolution Microscope

Nikon AX R Confocal Super-Resolution Microscope (currently Dept of Mol. Biol and Micro users only) and analysis workstation

Imaris Image Visualization and Analysis Workstation (version 9.3)

QuantStudio 3 Taqman PCR

BD LSR Fortessa Flow Cytometer (formerly was part of CFAR Immune Function Core)

Sony MA900 Cell Sorter (formerly was part of CFAR Immune Function Core)

You can view the calendars for this equipment in one of two ways.  If you are not logged in, use the link at the bottom of this screen.  If you are logged in, go to the "Schedule Equipment" tab.  You must be logged in to reserve time. If you do not have an account, you can go to the following website and request a login: https://my.ilabsolutions.com/account/signup/76?sc_id=2972

Services

Biosafety Level 3 Facility (for MTB and SARS CoV2 work)

Biosafety Level 2+ Facilities (for HIV work)

HIV Propagation                                              

Lentivirus or Pseudovirus Production

Luciferase Assay  

p24 ELISA                                                                                    

Virus Concentration

Latently infected Th17 cell production

EDITS (Env Detection of Induced Transcripts with Sequencing)

Deep Sequencing

DeltaVision Deconvolution Microscopy Training

High-Throughput Flow Cytometry Analysis

To request access to the BL-3 or BL-2+ Facilities or request a service, please click on the "Request Services" tab.  You must be logged in to see the tabs. If you have not received an email with your user name and password, you can go to the following website and request a login: https://my.ilabsolutions.com/account/signup/76?sc_id=2972

CFAR Assistance

If you have questions about the billing information that is required or other questions concerning requesting services at the core facility, please email iLabsCFAR@case.edu

Location and hours of operation

Virology: CWRU Biomedical Research Building, Room 1048;

NGS: 7100 Euclid Ave., Suite 190, Cleveland, OH;

Imaging: CWRU Wood Research Tower, Room 205a.

Getting Started 

1. Sign up for an iLab account by going to the Virology Sign-Up Page
2. If you have an iLab account but forgot your password, please click HERE
3. To access an instruction manaul, go to the iLab Customer Manual
   
4. To access the Principle Investigator instruction manual, go to the PI Training Manual
   

Contacts

Available Equipment and Resources