iLab Organizer :: Applied Functional Genomics Core

Overview of Services

The Applied Functional Genomics Core (AFGC) offers functional genomic study design and a highly standardized, top-to-bottom platform for conducting RNA isolation, Illumina-based RNA sequencing, and bioinformatics on a wide variety of clinical sample types.

We provide workflows that are optimized for many difficult sample types, such as ultra-low input (biopsies, laser capture, small cell numbers), 10X Genomics single cell-multiomic sequencing (scRNA-/snRNA-/ATAC-Seq), and Spatial Profiling via 10X Genomics' Visium HD/CytAssist and Nanostring's GeoMx/nCounter platforms.

Our analysis tools can integrate multiple data types, whether clinical or functional, in the bioinformatics analysis to uncover biomarkers or correlates. Our advanced bioinformatics methods include pathway, network, meta-analysis and drug discovery for researchers who require a look at biology across different studies, especially in correlation with clinical and immunophenotyping data.

The Applied Functional Genomics Core also serves as an assay and analytics platform for the Case Comprehensive Cancer Center's Integrated Genomics Shared Resource and the Rustbelt CFAR Biostatistics and Systems Biology Core (BSB). The CFAR BSB Core also backs the Inter-CFAR Integrated Analysis Group to support a unique partnership between the Rustbelt CFAR and the University of Miami CFAR, thus providing access to other omic technologies and meta-analysis for external CFAR members, and data integration.

Location/Shipping Address

AFGC
Case Western Reserve University School of Medicine
2109 Adelbert Road
Biomedical Research Building, Room 909B (North Labs)
Cleveland, OH 44106
Phone: (216) 368-3196

Contacts

Name	Role	Phone	Email	Location
Mark Cameron	Director	216-368-3196	mark.cameron@case.edu	BRB 926
Cheryl Cameron	Co-Director	440-991-7899	cheryl.cameron@case.edu	BRB 936
Banu Tamilselvan	Staff-Senior Genomics Research Associate (RNASeq Platforms)	216-233-7958	banumathi.tamilselvan@case.edu	BRB 947B
Leah Zagore	Staff-Senior Genomics Research Associate (Spatial Platforms)	216-368-8748	leah.mccord@case.edu	BRB 909B9
Brian Richardson	Staff-Senior Bioinformatics Associate	216-368-4662	brian.richardson2@case.edu	BRB 909B7

Name	Description	Price
Custom Single Cell Library Generation Request 10X Genomics	This option is available for custom requests, i.e. projects with special handling requirements, combined use of multiple platforms, etc. For example if you require the use of a barcoded antibody or custom probes with single cell sequencing. *Please note that unless otherwise stated, these prices do not include upstream sample processing/isolation or fixation work and price only includes the cost of library generation.* *Please request a quote for detailed pricing.* Prices are subject to change based on the number of samples multiplexed and type of kit used (ie 1X4, 4x4, or 4x16). Please also note that the sequencing cost is not included in any of these prices, and all sequencing will need to be performed externally at a separate core, which we can discuss during consultation. While we don't perform the sequencing in our core, we want you to be aware that the cost of sequencing will also vary widely based on the numbers of samples, type of platform, and sequencing depth you require.	Internal $1.00 each External - Academic $1.00 each External - Foundation/Non-profit $1.00 each External - Industry $1.00 each
Single Cell Multiome ATAC + Gene Expression (10X Genomics)	Includes transcriptome library prep, AND ATAC-Seq library prep.	Inquire

▼ ► Library Prep (2)
Name	Description	Price
Single Cell Multiome ATAC + Gene Expression (10X Genomics)	Includes transcriptome library prep, AND ATAC-Seq library prep.	Inquire
Custom Single Cell Library Generation Request 10X Genomics	This option is available for custom requests, i.e. projects with special handling requirements, combined use of multiple platforms, etc. For example if you require the use of a barcoded antibody or custom probes with single cell sequencing. *Please note that unless otherwise stated, these prices do not include upstream sample processing/isolation or fixation work and price only includes the cost of library generation.* *Please request a quote for detailed pricing.* Prices are subject to change based on the number of samples multiplexed and type of kit used (ie 1X4, 4x4, or 4x16). Please also note that the sequencing cost is not included in any of these prices, and all sequencing will need to be performed externally at a separate core, which we can discuss during consultation. While we don't perform the sequencing in our core, we want you to be aware that the cost of sequencing will also vary widely based on the numbers of samples, type of platform, and sequencing depth you require.	Internal $1.00 each External - Academic $1.00 each External - Foundation/Non-profit $1.00 each External - Industry $1.00 each

Map

and