Welcome!
ALERT Event: Nanoscopy Imaging Using STED 3X Technology – Achieving Resolutions Down to 30nm (Registry Required)
Date: Friday, Nov. 22nd. Time: 11:00 AM – 12:00 PM
Location: Biomedical Research Building, Room 105
Overview:
The seminar will cover the fundamentals and capabilities of STED 3X Nanoscopy and Confocal Super-Resolution technology. STED (Stimulated Emission Depletion) allows for super-resolution imaging purely through optical means, built on a confocal platform. Using the Leica TCS SP8 system, you can achieve 30nm XY and 130nm Z resolutions in STED mode, as well as 140nm XY and 300nm Z resolutions in confocal super-resolution mode, all powered by Leica’s exclusive White Light Laser (tunable from 470–670 nm) in a single system.
Hands-On Training (Registry Required):
Following the seminar, we'll be hosting four one-hour training sessions for STED users from 1:00 PM to 5:00 PM. Due to time constraints, each session will be limited, but it's a fantastic opportunity for our local imaging community.
Please register here. We look forward to seeing you there!
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The Applied Functional Genomics Core (AFGC) offers functional genomic study design and a highly standardized, top-to-bottom platform for conducting RNA isolation, Illumina-based RNA sequencing, and bioinformatics on a wide variety of clinical sample types.
We provide workflows that are optimized for many difficult sample types, such as ultra-low input (biopsies, laser capture, small cell numbers), 10X Genomics single cell-multiomic sequencing (scRNA-/snRNA-/ATAC-Seq), and Spatial Profiling via 10X Genomics' Visium CytAssist and Nanostring's GeoMx/nCounter platforms.
Our analysis tools can integrate multiple data types, whether clinical or functional, in the bioinformatics analysis to uncover biomarkers or correlates. Our advanced bioinformatics methods include pathway, network, meta-analysis and drug discovery for researchers who require a look at biology across different studies, especially in correlation with clinical and immunophenotyping data.
The Applied Functional Genomics Core also serves as an assay and analytics platform for the Case Comprehensive Cancer Center's Integrated Genomics Shared Resource and the Rustbelt CFAR Biostatistics and Systems Biology Core (BSB). The CFAR BSB Core also backs the Inter-CFAR Integrated Analysis Group to support a unique partnership between the Rustbelt CFAR and the University of Miami CFAR, thus providing access to other omic technologies and meta-analysis for external CFAR members, and data integration.
Location/Shipping Address
AFGC
Case Western Reserve University
2109 Adelbert Rd.
Wood Research Tower, RT100-5
Cleveland OH
44106-2624
Tel: 216-233-7958
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Mark Cameron |
Director
|
216-368-3196
|
mark.cameron@case.edu
|
Wood Building W113C
|
| Cheryl Cameron |
Co-Director
|
440-991-7899
|
cheryl.cameron@case.edu
|
BRB 936
|
| Banu Tamilselvan |
Staff-Senior Genomics Research Associate (RNASeq Platforms)
|
216-233-7958
|
banumathi.tamilselvan@case.edu
|
RT100-5 Research Tower Wood Building
|
| Leah Zagore |
Staff-Senior Genomics Research Associate (Spatial Platforms)
|
216-368-8748
|
leah.mccord@case.edu
|
W113A
|
| Brian Richardson |
Staff-Senior Bioinformatics Associate
|
216-368-4662
|
brian.richardson2@case.edu
|
WRB-1210
|
| Carmen Nichols |
Core Manager
|
216-368-8748
|
carmen.nichols@case.edu
|
W113B
|
| Service List |
| ► Library Prep (2) | |||
| Name | Description | Price | |
|---|---|---|---|
| Single Cell Fixed RNA Profiling (10X Genomics) |
Services include thawing, fixing and preserving cells, followed by library preparation and pooling. ***Please request a quote for detailed pricing.*** Prices are subject to change everytime based on the number of samples multiplexed and type of multiplexing kit used (ie 1X4, 4x4, or 4x16). Please also note that the sequencing cost is not included in any of these prices, and all sequencing will need to be performed externally at a separate core, which we can discuss during consultation. While we don't perform the sequencing in our core, we want you to be aware that the cost of sequencing will also vary widely based on the numbers of samples and sequencing depth you require. |
Inquire | |
| Single Cell Multiome ATAC + Gene Expression (10X Genomics) |
Includes transcriptome library prep, AND ATAC-Seq library prep. |
Inquire | |
| ► GeoMx (1) | |||
| Name | Description | Price | |
| GeoMx Slide Preparation and Scan |
Slide preparation and binding of barcoded antibodies/probes, GeoMx slide scan |
Inquire | |
