Bioanalyte Core at CWRU

Overview of Services

Bioanalyte Core Services started as a cytokine core about 15 years ago. It has been fostered into being a Core that focuses on bioanalytes from quantification, to synthesis, to function. The bioanalytes cover not only cytokines but any molecule that requires quantification such as enzymes, sugars, etc. We provide the nteractive development of the bioanalysis of the molecule of interest in the context of the studies of interest. Each client has the opportunity to meet with the core director to explain the project and overall goals so that the assays that are utilized for the evalaution can be optimized for the greatest possibiity of success. The agreement of the analaytes is then followed by our staff reaching out for specific quotes and getting our clients the best deal on their assays. We do extensive evaluation of each study to assure we understand the samples and how to best run them analytically. Samples are logged into the system, how we will analyze them is detailed followed by planning the study. 

Product List and assay information

• Luminex Assay Principle 
• Luminex assays and high performance assays - R&D Systems, a Bio-Techne brand
• Luminex Multiplexing Information - MilliporeSigma
• ThermoFisher Luminex Information
• Real Time Openarray
• Real Time PCR Assays

     ↓ Core equipment ↓

• Luminex 100/200
• MAGPIX
• QuantStudio 3
• Attune NxT Flow Cytometer
• Varioskan Lux Multimode Microplate Reader
• EVOS™ M5000 Imaging System
• SpectraMax i3x user guide
• VERSAMaxTunable Microplate Reader

 

• Benefits of Luminex Multiplex Assays

  • Small Sample Volume: Conserves sample by measuring multiple analytes in a single well with less than 50 µL of sample.
  • Save Time and Money: Save resources when compared to running multiple single-analyte ELISAs.
  • Flexibility: Build analyte panels to fit your research needs.
  • Low Variability: Minimizes experimental variability with multiple data points derived from a single experiment.

 

Location and hours of operation

Hours	Location
Monday - Friday 9:00 AM - 5:00 PM	BRB 809 BRB 811

 

Shipping address

Case Western Reserve University
2109 Adelbert Rd.
Biomedical Research Building (BRB)
Room 809
Cleveland, Ohio 44106-2624

 

Links and Resources

1. General iLab workflow PDF
2. Link to where to apply for funding

Contacts

Name	Role	Phone	Email	Location
Tracey L. Bonfield	Core Director	216-368-4558	tracey.bonfield@case.edu	BRB 822
David Fletcher	Core Manager	216-368-4916	david.fletcher@case.edu	BRB 809
John Dunn	Staff-Managing Scientist	216-368-5146	john.dunn@case.edu	BRB 8th floor Admin East
Evan Rudo	Assistant Manager	440-864-0547	exr234@case.edu	BRB 809

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