Welcome!
ALERT Event: Nanoscopy Imaging Using STED 3X Technology – Achieving Resolutions Down to 30nm (Registry Required)
Date: Friday, Nov. 22nd. Time: 11:00 AM – 12:00 PM
Location: Biomedical Research Building, Room 105
Overview:
The seminar will cover the fundamentals and capabilities of STED 3X Nanoscopy and Confocal Super-Resolution technology. STED (Stimulated Emission Depletion) allows for super-resolution imaging purely through optical means, built on a confocal platform. Using the Leica TCS SP8 system, you can achieve 30nm XY and 130nm Z resolutions in STED mode, as well as 140nm XY and 300nm Z resolutions in confocal super-resolution mode, all powered by Leica’s exclusive White Light Laser (tunable from 470–670 nm) in a single system.
Hands-On Training (Registry Required):
Following the seminar, we'll be hosting four one-hour training sessions for STED users from 1:00 PM to 5:00 PM. Due to time constraints, each session will be limited, but it's a fantastic opportunity for our local imaging community.
Please register here. We look forward to seeing you there!
(hide this warning on this page)Prices listed below apply to Internal and External non-profit customers.
External for-profit customers will be subject to prices as listed on the services page.
| Overview of Services | |||||
| Service / Expertise | Cost | Description | |||
| Consultation and Data Management | |||||
| Protocol startup | $300.00 | Identification of relevant laboratory correlates; development of instructions and SOPs; data entry and tracking | |||
| Consult/Supplemental Technical Support | $36.00/hr | Per hour charge for consultation, sample movement, etc. | |||
| Cytokine and Multiplex Analyses | |||||
| Single Target ELISA | $220 + Kit | Performance and analysis of 1 plate; up to 32 specimens, in duplicate | |||
| Ella | $150.00 + kit | Performance and analysis of 1 plate, up to 70 specimens | |||
| Preclinical Drug Testing | |||||
| Growth Inhibition/Cytotoxicity Assessment (1 drug/1 cell line) | $95.00 | 1 drug on 1 cell line, 10 doses, 5 replicates | |||
| Growth Inhibition/Cytotoxicity Assessment non-adherent cells (1 drug/1 cell line) | $135.00 | MTT assay: 1 drug on 1 cell line, 10 doses, 5 replicates | |||
| Growth Inhibition/Cytotoxicity Assessment (2 drugs/1 cell line or 1 drug/2 cell lines) | $130.00 | 2 drugs, 10 doses each, in triplicate on 1 cell line or 1 drug 10 doses in triplicate on 2 cell lines | |||
| Growth Inhibition/Cytotoxicity Assessment non-adherent cells (2 drugs/1 cell line or 1 drug/2 cell lines) | $150.00 | MTT assay: 2 drugs, 10 doses each, in triplicate on 1 cell line or 1 drug 10 doses in triplicate on 2 cell lines | |||
| Mycoplasma Testing | $33.00 | MycoAlert™ detection method | |||
| SpectraMax Use | $27.00 | Per hour (0.5 hour minimum) in 30min increments | |||
| SpectraMax Use (after Hours) | $17.00 | Before 8am or after 5pm and weekends | |||
| Seahorse Use | $18.00 | Per Hour, 4 hr minimum charge | |||
| Lab Analysis | |||||
| Comet (Single Cell DNA damage) Assay | $60.00 | Minimum of 10 specimens, controls provided by TRSR | |||
| Cell Cycle analysis (PI or DAPI stain) | $65.00 | PI or DAPI staining and analysis, per group. Minimum 4 specimens | |||
| S Phase Cell Cycle analysis (EdU incorporation) | $75.00 | EdU staining / S phase analysis, per group, Minimum of 4 specimens | |||
| Flow Cytometry Processing, Antibody Stain | $120.00 | Minimum 4 specimens, up to 2 targets, additional targets extra | |||
| Western Blot | |||||
| 1 gel 1 membrane | $175.00 | Additional charge for antibodies and/or additional probes | |||
| 1 gel split membrane | $200.00 | Additional charge for antibodies and/or additional probes | |||
| 2 gels 1 membrane each | $275.00 | Additional charge for antibodies and/or additional probes | |||
| 2 gels split membranes | $300.00 | Additional charge for antibodies and/or additional probes | |||
| Nucleic Acid Isolation | |||||
| Total Nucleic Acid Isolation (AllPrep), Blood or Cultured Cells | $60.00 | Per Specimen | |||
| Total Nucleic Acid Isolation (AllPrep), Tissue | $70.00 | Per Specimen | |||
| RNA Isolation, Blood or Cultured cells | $50.00 | Per Specimen | |||
| RNA Isolation, Tissue | $60.00 | Per Specimen | |||
| Mammalian DNA Isolation, Blood or cultured cells | $42.00 | Per Specimen | |||
| Mammalian DNA Isolation, Tissue or Stool | $45.00 | Per Specimen | |||
| Bacterial/Microbiome DNA Isolation | $30.00 | Per Specimen | |||
| Nucleic Acid Quantitation | $4.00 | Nanodrop or Qubit method | |||
| Tapestation: DNA D1000 |
This service has move to the Genomics Core |
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| Tapestation: DNA D5000 |
|
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| Tapestation: RNA |
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| Tapestation: RNA HS | $30 set up fee + $10 per specimen | experienced users may request to run their own tapes $25/run | |||
| RNA/DNA Repurification | $33.00 | Repurification for Analysis | |||
| Clinical Sample Processing | |||||
| Routine Sample Processing | $100/visit | Includes routine processing, storage and ambient shipping of up to 5 tubes acquired in a single day, PBMC isolation not included | |||
| Additional Sample Processing | $22/tube | Charge for tubes above 5 acquired during a visit, PBMC not included | |||
| Histology Biopsy | $50.00 | Per shipment. Includes picking up samples from Tissue Resource core, sendout,tracking | |||
| PBMC Isolation, single timepoint | $120.00 | CPT or Ficoll-Hypaque Method including cryopreservation, if required | |||
| Pathology Request | $145.00 | Per specimen, Coordination of acquisition, tracking, shipping of slides or blocks required for clinical trials | |||
| Flow Cyt Prep/Formalin Fixation | $300.00 | Per visit, multiple spins and formalin fixation for flow cytometry | |||
| Biorepository Processing | $50.00 | Tracking and on site storage of a single biospecimen | |||
| On Campus Delivery | $40.00 | Delivery of specimens to facilities on UH/CWRU campus | |||
| Tier 2 Routine Processing base rate | $125.00 | To be used for more intense protocols with highter impact than normal processing or shipping requirements | |||
| Shipping charges | $12.00-$65.00 | Additional charges for trials that do not provide airbills/account numbers. Price depends on service | |||
| Location and hours of operation | |||||
| Hours | Location | ||||
| 8AM-6PM | 11100 Euclid | ||||
| Seidman CC, Rm. 3608 | |||||
| Cleveland, OH. 44106 | |||||
| 9AM-5PM | 2103 Cornell Rd. | ||||
| Wolstein Reasearch Building, Rm. 3503 | |||||
| Cleveland, OH. 44106 | |||||
| Links and Resources | |||||
| Translational Research | |||||
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Erin Hohler |
Research Assistant - UH
|
216-286-3889
|
erin.hohler@case.edu
|
Seidman CC, Rm. 3608
|
| Rebecca Fuller |
Research Assistant - Wolstein
|
216-368-1141
|
rebecca.fuller2@case.edu
|
Wolstein Research Building Rm 3503
|
| Jessica Trabucco |
Research Assistant - UH
|
216-286-3890
|
jessica.trabucco@case.edu
|
Seidman CC, Room 3608
|
| John Pink |
Core Director
|
216-368-5420
|
john.pink@case.edu
|
| Service list |
| ► Consultation and Data Management (2) | |||
| Name | Description | Price | |
|---|---|---|---|
| Consult/Supplemental Technical Support |
|
Internal
$36.00
/hr
External - Academic $36.00 /hr |
|
| Protocol startup |
Identification of relevant laboratory correlates; development of instructions and SOPs; data entry and tracking |
Inquire | |
| ► Clinical Sample Processing (15) | |||
| Name | Description | Price | |
| Additional Sample Processing |
Charge for tubes above 5 acquired during a visit, PBMC not included |
Inquire | |
| Ambient 2-day delivery |
Charge for shipment of pathology samples that don't require an overnight delivery |
Inquire | |
| Ambient Overnight Delivery |
Charge for shipment of processed samples ambient overnight (no airbill provided by study) |
Inquire | |
| Biorepository Processing |
Tracking and on site storage of a single biospecimen, no processing |
Inquire | |
| Courier to UH Satellites |
Charge for using Fedex Sameday to transfer samples/materials to the UH Satellite institutions |
Inquire | |
| Histology Biopsy |
Per shipment. Includes pickup from Tissue Resource Core, sendout and tracking. |
Internal
$50.00
each
External - Industry $74.00 each External - Academic $50.00 each |
|
| Local Courier to CCF |
Use of Fedex Sameday service to transfer samples/materials to Cleveland Clinic |
Inquire | |
| On Campus Delivery |
Delivery of specimens to facilities on UH/CWRU campus |
Inquire | |
| Pathology Request |
Per specimen, Coordination of acquisition, tracking, shipping of slides or blocks required for clinical trials |
Inquire | |
| PBMC Isolation, single timepoint |
CPT or Ficoll-Hypaque Method including cryopreservation, if required |
Inquire | |
| Purchasing for protocol |
Ordering specific items for specific protocols that we don't want to pay for |
Inquire | |
| Refrigerated/Frozen Overnight shipment surcharge |
Additional charge for processed samples needing overnight shipments refrigerated or on dry ice (unless Airbill is included) |
Inquire | |
| Routine Sample Processing |
Includes routine processing, storage and ambient shipping of up to 5 tubes acquired in a single day, PBMC isolation not included |
Inquire | |
| Saturday Delivery Surcharge |
Charge in addition to other shipment charges for packages that must be delivered on a Saturday |
Inquire | |
| Tier 2 Routine Processing base rate |
This is a routine processing charge for more intense protocols, with extra charges to account for more intense processing and/or shipping requirements |
Inquire | |
| ► Cytokine and Multiplex Analyses (3) | |||
| Name | Description | Price | |
| ELLA |
Cost of kit NOT included |
Inquire | |
| Single Target ELISA |
Performance and analysis of 1 plate; up to 32 specimens, in duplicate. Cost of kit NOT included |
Inquire | |
| Western Blot |
Pricing determined based on specific project requirements, to be determined after consultation Starting price $175 |
Internal
$175.00
each
External - Academic $175.00 each External - Foundation/Non-profit $175.00 each External - Industry $262.50 each |
|
| ► Instrument Charges (5) | |||
| Name | Description | Price | |
| Mesoscale Instrument Use |
Per use. Trained users only |
Inquire | |
| Seahorse cartridges | Inquire | ||
| Seahorse XFe96 Analyzer |
per hour, 4 hr maximum charge |
Inquire | |
| SpectraMax Use after hours per 30minute intervals |
Before 8am or after 5pm and weekends, charge per 30minutes |
Inquire | |
| Training plates |
Seahorse plates for training purposes |
Inquire | |
| ► Lab Analysis (2) | |||
| Name | Description | Price | |
| Cell Cycle analysis (PI or DAPI stain) |
PI or DAPI staining and analysis, per group. Minimum 4 specimens |
Inquire | |
| Comet (Single Cell DNA damage) Assay |
Minimum of 10 specimens, controls provided by TRSR |
Inquire | |
| ► Nucleic Acid Isolation (6) | |||
| Name | Description | Price | |
| Nucleic Acid Sample Desalting |
Internal
$10.00
each
External - Academic $10.00 each External - Foundation/Non-profit $10.00 each External - Industry $10.00 each |
||
| Bacterial/Microbiome DNA Isolation |
Per Specimen |
Internal
$30.00
each
External - Academic $30.00 each External - Foundation/Non-profit $30.00 each External - Industry $43.50 each |
|
| Nucleic Acid Quantitation |
Nanodrop or Qubit method per sample |
Internal
$4.00
each
External - Academic $4.00 each External - Foundation/Non-profit $4.00 each External - Industry $5.75 each |
|
| Tapestation: DNA Set Up Fee |
Tape Station has move to Genomics Core Please contact Rebecca Fuller at rxf182@case.edu if you need assistance Set up fee for DNA tests on the Tapestation
|
Inquire | |
| Tapestation: RNA Set Up Fee |
Tape Station has move to Genomics Core Please contact Rebecca Fuller at rxf182@case.edu if you need assistance Set up fee for RNA test on the Tapestation *NOT for HS RNA |
Inquire | |
| Tapestation: HS RNA Set Up Fee |
Tape Station has move to Genomics Core Please contact Rebecca Fuller at rxf182@case.edu if you need assistance Set up fee for HS RNA test on the Tapestation |
Inquire | |
